Alteration in the Cerebrospinal Fluid Lipidome in Parkinson’s Disease: A Post-Mortem Pilot Study

Lipid metabolism is clearly associated to Parkinson’s disease (PD). Although lipid homeostasis has been widely studied in multiple animal and cellular models, as well blood derived from PD individuals, the cerebrospinal fluid (CSF) lipidomic profile remains largely unexplored. In this study, we characterized post-mortem CSF imbalance between neurologically intact controls (n = 10) subjects 20). The combination of dual extraction with ultra-performance liquid chromatography-electrospray ionization quadrupole-time-of-flight mass spectrometry (UPLC-ESI-qToF-MS/MS) allowed for monitoring 257 species across all samples. Complementary multivariate univariate data analysis identified that glycerolipids (mono-, di-, triacylglycerides), saturated mono/polyunsaturated fatty acids, primary amides, glycerophospholipids (phosphatidylcholines, phosphatidylethanolamines), sphingolipids (ceramides, sphingomyelins), N-acylethanolamines sterol lipids (cholesteryl esters, steroids) were significantly increased compared control group. Interestingly, dyshomeostasis differed depending on neuropathological staging duration. These results, despite limitation being obtained a small population, suggest extensive remodeling PD, shedding new light deployment lipidomics promising tool identify potential markers discriminatory other atypical parkinsonisms.